Supplemental Figures 1-7 from Chemotherapeutic Sensitivity of Testicular Germ Cell Tumors Under Hypoxic Conditions Is Negatively Regulated by SENP1-Controlled Sumoylation of OCT4

Fig. S1 Expression of OCT4 in NCCIT cells under normoxia and hypoxia. Fig. S2 Effect of increasing EGFP-SUMO1gg concentrations on endogenous OCT4 protein levels in NCCIT cells under normoxia. Fig. S3 Transcriptional expression levels of endogenous SENP1 in human embryonic stem (hES), NCCIT, and NT2 cells by a quantitative real-time PCR analysis. Fig. S4 Effect of SENP1 on endogenous OCT4 protein stability in NCCIT cells using a Western blotting analysis. Fig. S5 Effects of hypoxia and SENP1 on the viability of NCCIT cells using a WST-1 assay. Fig. S6 Expressions of HIF-1α, HIF-2α, and OCT4 in pluripotent human embryonic stem (hES) and NCCIT cells. Fig. S7 Effect of hypoxia on the SENP1 and RSUME expression level in NCCIT cells.