Figure S3 from The Small GTPase ARF6 Activates PI3K in Melanoma to Induce a Prometastatic State

Figure S3. Arf6Q67L-HA expression in tumors from BrafCA;Cdkn2af/f;Ptenf/f mice. qRT-PCR detection of Arf6 Q67L-HA from fresh, frozen primary tumor fragments. Bars represent the absolute difference in crossing threshold (Ct) between Arf6 Q67L-HA and Gapdh. The Ct threshold that defines detection was established above the background signal for controls in both the BrafCA;Cdkn2af/f and the BrafCA;Cdkn2af/f ;Ptenf/f cohorts (see also Figure S1). All mouse tumor negative by qRT-PCR showed detectable HA-tagged ARF6Q67L by immunohistochemistry (not shown, see Figure S1 for example).