Supplementary Figure 1 from Soluble CD200 Is Critical to Engraft Chronic Lymphocytic Leukemia Cells in Immunocompromised Mice

PDF file - 105K, CD200 shedding by CLL cells: Detection of sCD200 in CLL supernatant: a) CLL cells isolated from peripheral blood of patients were cultured in AIM-V medium. Supernatants were collected at 24 and 48hr time points and assessed for sCD200 level by CD200 ELISA. Variable levels of sCD200 were detected in CLL supernatants in a time dependent manner, suggesting constitutive release of sCD200. b) Treatment of CLL cells with the global protease inhibitor GM6001 at a final concentration of 20muM inhibited sCD200 shedding. Error bars are indicative of standard deviation of sCD200 levels detected in two independent ELISAs