Supplementary Materials from Endothelial Cell Protein C Receptor Opposes Mesothelioma Growth Driven by Tissue Factor

Supplementary File PDF 277K, Supplementary Methods: Includes additional details of reagents and methodology, and references. Supplementary Figure 1: Effect of FVIIa and thrombin on cell proliferation of wild-type REN, TF- or PAR1- knocked-down REN MPM cells. Supplementary Figure 2: TF- or PAR1 knock-down in REN MPM cells suppresses in vivo tumor cell proliferation. Supplementary Figure 3: TF- or PAR1 knock-down in REN MPM cells increases tumor cell apoptosis in vivo. Supplementary Figure 4: Over expression EPCR did not alter in vitro growth potential of REN MPM cells. Supplementary Figure 5: Tissue factor pathway inhibitor (TFPI) and thrombomodulin (TM) protein levels in parental MS-1 and M9K MPM cells and their variants as analyzed by western blot analysis. Supplementary Figure 6: EPCR knock-down did not alter cell proliferation of MS-1 or M9K MPM cells in vitro