Supplemental Methods from Metabolic Vulnerabilities in Endometrial Cancer

Frances L. Byrne,Ivan K.H. Poon,Susan C. Modesitt,Jose L. Tomsig,Jenny D.Y. Chow,Marin E. Healy,William D. Baker,Kristen A. Atkins,Johnathan M. Lancaster,Douglas C. Marchion,Kelle H. Moley,Kodi S. Ravichandran,Jill K. Slack-Davis,Kyle L. Hoehn

crossref（2023）

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Abstract

Supplemental Methods. Includes more detailed methods for the main manuscript and additional supplemental methods including Cell Culture, Sources of drugs/radioactive tracers,Targets/functions of metabolic drugs and antioxidants, Preparation of protein lysates, Nutrient metabolism assays, qPCR primer sequences, Cytotoxicity assays, Cell doubling times, Cellular ATP levels, ROS assays, Mass spectrometry of Acetyl-CoA and Pyruvylated-CoA, and Supplemental References.

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