Unlocking thioester-mediated biocatalysis with in situ cofactor recycling

Thioesters, especially those derived from coenzyme A, are ubiquitous biological intermediates, yet accessing them synthetically is difficult and costly. In Nature Catalysis , Flitsch et al. describe a novel recycling system that regenerates thiol cofactors in situ and provides a general biocatalytic strategy for one-pot functionalization of carboxylic acids.