High-throughput Biological Microarrays Based on Framework Nucleic Acids

A high-throughput biological microarray based on framework nucleic acid (FNA) was constructed. Using an automated ultra-micro pipetting platform, the droplets containing FNAs-based probes were fabricated onto the glass substrates according to the pre-defined commands. After the targets were captured by the probes, the microarrays were scanned by the integrated gene chip scanner, the results of which could be further used for the quantification of target concentration. It was found that the FNA probes could be prepared with high throughput on the biological microarrays, and the microarrays with 150000 spots can be prepared within 24 h. Moreover, the relative deviation (W) of distance between the points is less than 10%, and the coefficient of variation (CV) of fluorescence intensity is 3.30%. The properties of each spot on the microarrays were stable, which is much higher than the national standard. Additionally, we demonstrated that the FNA-based microarray possessed high addressability, high sensitivity and high-throughput capabilities. The limit of detection for nucleic acid target was down to 100 pmol/L. With the progress of various probe technologies, the microarray platform shows great potential for bioanalysis.