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Conversion of endogenous phenolic acid in brown rice by bioextrusion of mesophilic -amylase

Yu Qin, Shuohan Ma, Jianwei Zhou, Dandan Li, Jianle Chen, Wenjun Wang, Huan Cheng, Zhengzong Wu, Jinhu Tian, Enbo Xu, Donghong Liu

Food Bioscience(2023)

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Abstract
Thermomechanical process is the most commonly used for treating grains but inevitably cause loss of endoge-nous bioactive ingredients such as phenolics. This work investigated the phenolic retention of brown rice via bioextrusion at different concentration gradients of mesophilic alpha-amylase (M alpha A, 0-2000 U/g), and determined the conversion of individual phenolic acids at both free and bound forms. Results showed that extrusion pro-moted the release of phenolics but lead to a great loss of them. While the phenolic retention of brown rice was significantly increased with increasing M alpha A levels up to 1000 U/g (twice more than that of traditional extru-date), which simultaneously modified rice matrix from big granules to small ones by SEM observation. The viscosity of bioextruded brown rice was decreased significantly but negatively relating to phenolic retention. Extrusion kinetics revealed that mechanical input and residence time had obvious effects on starch gelatinization which ultimately influence the presence of phenolics.
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Key words
Whole grain,Extrusion,Free,bound phenolics,Starch enzymolysis,Kinetics,Correlation analysis
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