枳实化学成分提取分离及其抑菌活性初步研究
Yunnan Chemical Technology(2023)
Abstract
采用醇提、萃取、大孔树脂柱层析、硅胶柱层析、聚酰胺柱层析、水解、重结晶等方法从中药枳实中提取分离得到5种枳实化学成分.经核磁氢谱、高分辨质谱和文献数据对比,分别鉴定为桔皮素、橙皮苷、橙皮素、柚皮苷、柚皮素.采用菌落直径法首次测定了这5种枳实化学组分对茶叶轮斑病菌、辣椒疫霉病菌、茶叶炭疽病菌等10种植物病原真菌的抑菌活性.结果表明:5种枳实化学组分整体上抑菌活性较弱.但桔皮素对茶叶轮斑病菌、茶叶炭疽病菌、辣椒疫霉病菌都表现出一定的抑菌活性,抑制率分别为43.89%、61.54%、46.85%,值得进一步研究.
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Key words
Fructus Aurantii Immaturus,chemical components,extraction and separation,antibacterial activity
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