湖北'天仙红'桃树流胶病病原菌鉴定
Plant Protection（2023）
Abstract
流胶病在湖北'天仙红'桃树上发生严重.为明确引起'天仙红'桃流胶病的病原,通过形态学、分子生物学及柯赫氏法则对采集的病害样品进行了病原菌鉴定.结果表明,获得的分离菌株JSZ01的形态学特征与小新壳梭孢Neofusicoccum parvum基本一致.BLAST比对结果显示,分离菌株JSZ01的ITS、TEF1-α和β-tubulin基因序列与N.parvum的相似性在99.2％～100％,在系统发育进化树中JSZ01与小新壳梭孢的类群处于同一分支.JSZ01接种健康桃树枝条5 d后接种部位出现溃疡流胶症状,与田间症状一致.结合形态学观察、序列分析以及致病性测定结果,将'天仙红'桃树流胶病的病原菌鉴定为N.parvum.
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Key words
‘Tianxianhong’peach,gummosis,pathogen identification,Neofusicoccum parvum
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