橡胶狗尾草平脐蠕孢叶斑病病原菌鉴定及生物学特性研究
Plant Protection（2023）
Abstract
为明确采自西双版纳景洪地区疑似橡胶麻点病的病原菌,采用组织分离法进行病原菌分离,活体接种法进行致病性测定,通过形态学观察结合rDNA-ITS和GAPDH两个核苷酸片段序列分析,将病原菌确定为狗尾草平脐蠕孢Bipolaris setariae.通过单因子变量试验,对病原菌进行生物学特性研究,结果表明:菌丝生长最适温度为30℃,最适pH为7～8,最适培养基为PDA,菌丝致死温度为53℃,10 min.该病原菌能有效利用多种碳源和氮源,碳源以可溶性淀粉利用率最高,最适氮源为蛋白胨.24 h黑暗条件下培养更有利于菌丝生长.分生孢子萌发的最适温度为28～35℃,最适pH为7～8,28℃黑暗培养2 h后萌发率达到最大,为95.5％,分生孢子致死温度为60℃,10 min.
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Key words
rubber tree,leaf spot disease,Bipolaris setariae,biological characteristic
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