Biochemical Methods for Assessing Gasdermin D Inactivation in Macrophages.

Gasdermin D is an essential mediator of pyroptosis. Under resting conditions gasdermin D is inactive in the cytosol. Following inflammasome activation, gasdermin D undergoes processing and oligomerization to create membrane pores and induce pyroptosis to release mature IL-1 and IL-18. Biochemical methods for the analysis of gasdermin D activation states are important for assessing gasdermin D function. Here we describe the biochemical methods for assessing gasdermin D processing and oligomerization and its inactivation using small molecule inhibitors.