Multiclass method to determine emerging pollutants in bats using a non-invasive approach based on guano matrix

•Multi-class method to determinate emerging pollutants in bat wildlife.•Guano is an invasive matrix and relatively easy to collect without stressing the animals.•Sample treatment involving steps of liophilization, USE and d-SPE prior to LC–MS/MS.•Successful linearity, recoveries, and limits of quantification (from 0.01 to 64 ng/g dw).•Suitability of guano samples for exposure studies to emerging pollutants in wild animal.