Evaluation of a multiplexed oligonucleotide ligation assay for SARS-CoV-2 variant identification.

Daniel Solis,Mamdouh Sibai, Faith Kung, Timothy J Break,Seth B Harkins,ChunHong Huang,Fumiko Yamamoto,Malaya K Sahoo,Jacob N Wohlstadter,George B Sigal,Benjamin A Pinsky

Journal of clinical virology : the official publication of the Pan American Society for Clinical Virology（2023）

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摘要

This multiplexed oligonucleotide ligation assays demonstrated accurate SARS-CoV-2 variant typing compared to whole genome sequencing. Such an approach has the potential to provide improved turnaround compared to sequencing and more detailed mutation coverage than RT-qPCR.

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关键词

multiplexed oligonucleotide ligation assay,variant identification,sars-cov

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