Functional properties of individual sub-domains of the fibrin(ogen) αC-domains

•The use of specific proteases allowed us to obtain truncated forms of fibrin(ogen) lacking its C-terminal sub-domains or both N- and C-terminal sub-domains of their αC-domains. These fibrin(ogen) forms have been used for studying the role of the corresponding sub-domains in several fibrin(ogen)-dependent processes.•It was shown that the N- and C-terminal sub-domains both play an important role in fibrin polymerization. These two sub-domains also contributed to platelet aggregation with the N-terminal sub-domains playing a more significant role in this process.•It was also shown that the C-terminal sub-domains make the major contribution to the plasminogen activation process, endothelial cell viability, and migration of cancer cells.