Diuretic Activity of Flavonoids and Dense Extract of Spreading Marigold Flowers

Spreading marigold ( Tagetes patula L.) is a valuable source of biologically active compounds, including flavonoids. The aim of the present work was to study the diuretic activity of a dense extract from spreading marigold flowers and the dominant flavonoids of this type of raw material, i.e., patulitrin and patuletin. The two flavonoids patuletin (3,5,7,3′,4′-pentahydroxy-6-methoxyflavone) and patulitrin (3,5,7,3′,4′-pentahydroxy-6-methoxyflavone 7- O -β- D -glucopyranoside) were isolated using column chromatography over silica gel L 40/100 and were identified by UV, PMR, and 13 C NMR spectroscopy. Diuresis significantly increased in animals of the experimental group relative to the water control indicators (like the effect of the reference drug furosemide at a threshold dose of 1 mg/kg) with a single intragastric administration of the dense extract of marigold flowers at a dose of 10 mg/kg and patuletin and patulitrin at a dose of 1 mg/kg against a background of 3% water load in a 4-h experiment. Diuresis and creatininuresis (a sign of increased glomerular filtration) significantly increased by the end of a 24-h experiment with the dense extract (like the effect of hypothiazide at an effective average therapeutic dose of 20 mg/kg).