Supplementary Figure 1 from HIF1α Regulates mTOR Signaling and Viability of Prostate Cancer Stem Cells

Supplementary Figure 1. A) We identified a Lin-/CD49f+/Sca-1+ basal prostate stem cell population in wild-type C57BL/6 mice (left). HIF-1α mRNA was upregulated in sorted basal murine prostate stem cells (n=5, right). B) Treatment with increasing concentration of HIF-1α inhibitor leads to a dose dependent HIF-1α protein downregulation in hypoxic TRAMP-C1 cells. HIF-1α protein levels were assessed by immunoprecipitation and western blotting. IgG heavy chain (HC) is shown as a loading control for immunoprecipitation. Quantified and normalized protein levels are shown. C) HIF-1α shRNA expression in the DU145 cell line (shHIF1A) leads to reduced mRNA levels of HIF-1α and reduced expression of its target gene REDD1. Scrambled shRNA (scrCo) served as control. D) Knockdown of HIF-1α mRNA in the TRAMP-C1 murine cancer cell line. Scrambled shRNA (scrCo) served as control.