Supplementary Naterials and Methods. Supplmentary Figures 1-6 from Targeting the Proteasome-Associated Deubiquitinating Enzyme USP14 Impairs Melanoma Cell Survival and Overcomes Resistance to MAPK-Targeting Therapies

Supplementary Figure 1. b-AP15 and its derivative VLX1570 inhibit USP14 activity in melanoma cells. Supplementary Figure 2. USP14 inhibition irreversibly induces growth inhibition and cytotoxicity in melanoma cells. Supplementary Figure 3. USP14 overexpression reduces the cytotoxic effect of b-AP15 on melanoma cell. Supplementary Figure 4. siRNA-mediated depletion of USP14 reduces melanoma cell growth and focus formation. Supplementary Figure 5. USP14 targeting induces caspase-independent and ROS-dependent cell death. Supplementary Figure 6. USP14 inhibition induces a rapid accumulation of polyubiquitinated proteins and HSP70 in melanoma cells