Analysis of Arabidopsis venosa4-0 supports the role of VENOSA4 in dNTP homeostasis

An imbalance in the deoxyribonucleoside triphosphate (dNTP) pool caused by an increase or decrease in the levels of any of the four dNTPs leads to increased DNA mutations, overloading DNA repair mechanisms. The human protein SAMHD1 (Sterile alpha motif and histidine-aspartate domain containing protein 1) functions as a dNTPase to maintain the balance of the dNTP pool, as well as in DNA repair. In eukaryotes, the limiting step in de novo dNTP synthesis is catalyzed by RIBONUCLEOTIDE REDUCTASE (RNR), which consists of two R1 and two R2 subunits. In Arabidopsis, RNR1 is encoded by CRINKLED LEAVES 8 ( CLS8 ) and RNR2 by three paralogous genes, including TSO2 ( TSO MEANING 'UGLY' IN CHINESE 2 ). In plants, the de novo biosynthesis of purines occurs within the chloroplast, and DOV1 (DIFFERENTIAL DEVELOPMENT OF VASCULAR ASSOCIATED CELLS 1) catalyzes the first step of this pathway. Here, to explore the role of VENOSA4 ( VEN4 ), the most likely Arabidopsis ortholog of human SAMHD1 , we studied the ven4-0 mutant. The mutant leaf phenotype caused by the ven4-0 point mutation was stronger than those of T-DNA insertional ven4 mutations. Structural predictions suggested that the E249L amino acid substitution in the mutated VEN4-0 protein rigidifies its 3D structure compared to wild-type VEN4. The morphological phenotypes of the ven4 , cls8 , and dov1 single mutants were similar, and those of the ven4 tso2 and ven4 dov1 double mutants were synergistic. The ven4-0 mutant had reduced levels of four amino acids related to dNTP biosynthesis, including glutamine and glycine, which are precursors in the de novo purine biosynthesis pathway. Finally, despite its annotation in some databases, At5g40290, a paralog of VEN4 , is likely a pseudogene. These observations support the previously proposed role of VEN4 in dNTP metabolism. Our results reveal a high degree of cross-kingdom functional conservation between VEN4 and SAMHD1 in dNTP homeostasis. ### Competing Interest Statement The authors have declared no competing interest.