Supplementary Materials and Methods and Figures from Histone Acetyltransferase PCAF Is Required for Hedgehog–Gli-Dependent Transcription and Cancer Cell Proliferation

PDF file, 906K, Supplementary Materials and Methods Flow cytometry. Colony formation assay. Supplementary Tables Supplementary Table 1. siRNAs and shRNAs constructs used in this study. Supplementary Table 2. Expression primers and ChIP primers used in this study. Supplementary Table 3. Antibodies used in this study. Supplementary Figures Supplementary Figure S1. Analysis of siRNA screening for acetyltransferases. Supplementary Figure S2. Validation of candidate genes from the siRNA screening. Supplementary Figure S3. Stable depletion of Pcaf does not affect DNA synthesis in NIH3T3 cells. Supplementary Figure S4. Inhibition of PCAF expression affects neurosphere formation and proliferation of GBM543 cells. Supplementary Figure S5. Depletion of PCAF and inhibitors to PCAF induce apoptosis. Supplementary Figure S6. Inhibition of Pcaf expression affects proliferation of tNSCs.