Supplementary Figures from Tumor Cells Upregulate Normoxic HIF-1α in Response to Doxorubicin

PDF file, 257K, Western blot: doxorubicin-induced normoxic HIF-1� expression was compared to a positive control of HIF-1� induced by hypoxia (S1); Hoechst 33342 labeling: comparison of perfused tumor fraction in 4T1ODD-luc tumors on days 0, 1, 4, 7, and 16 post-treatment (n = 5, mean � SE). P > 0.05. Student t test (S2); Western blot: increasing concentration of doxorubicin led to higher HIF-1� expression 48 hours post-treatment (S3); WST-1 cell viability assay at 24, 48, and 72 hours post-treatment (S4), Western blot: the expressions of JAK2 and phosphorylated JAK2 (Tyr1007/1008) 48 hours post-treatment (S5); Quantification of iNOS band density which is normalized to loading control in Fig. 5D (S6); Comparison of TAM-specific marker CD68 fraction in 4T1ODD-luc tumors on days 0, 1, 4, 7, and 16 post-treatment (n = 5, mean � SE). P > 0.05, Student t test (S7).