Supplementary Figure 3 from Chk2 Phosphorylation of Survivin-ΔEx3 Contributes to a DNA Damage–Sensing Checkpoint in Cancer

PDF file - 21K, Pull down experiments. Recombinant GST, GST-survivin (SVV) or GST-survivin-DeltaEx3 was incubated with LN229 total cell extracts (TCE) and bound proteins were analyzed with an antibody to Chk2 (A) or Hsp60 (B) by Western blotting. Recombinant proteins used for the pull down experiments were stained with Coomassie blue (bottom panels).