Supplementary Figures 1-12 from The CRTC1-NEDD9 Signaling Axis Mediates Lung Cancer Progression Caused by <i>LKB1</i> Loss

PDF file - 307K, Supplementary Figure S1. Ectopic NEDD9 expression partially reverses the inhibitory function of LKB1 upon cell migration and invasion of human lung cancer cells. Supplementary Figure S2. shNedd9 inhibits lung cancer progression in de novo KrasG12D, Lkb1L/L mouse model. Supplementary Figure S3. NEDD9 promotes lung cancer progression in de novo KrasG12D mouse model. Supplementary Figure S4. LKB1 specifically regulates CRE-Luc activity. Supplementary Figure S5. shCRTC1 down-regulates NEDD9 mRNA expression. Supplementary Figure S6. Screening for the responsive element of CRTC1/CREB on NEDD9 promoter. Supplementary Figure S7. LKB1 regulates NEDD9 promoter activity through the non-classical CRE site. Supplementary Figure S8. Immunofluorescence assay showed that ectopic Flag-LKB1 expression promoted SIK2 translocation into nucleus in A549 cells. Supplementary Figure S9. Immunofluorescence assay showed that LKB1 knockdown promoted SIK2 translocation into cytoplasm while CRTC1 translocation into nucleus in CRL-5866 cells. Supplementary Figure S10. SIK2 knockdown up-regulates NEDD9 mRNA expression via promoting CRTC1 nuclear translocation. Supplementary Figure S11. Ectopic CRTC1 expression partially reverses the inhibitory function of LKB1 upon cell migration and invasion of human lung cancer cells. Supplementary Figure S12. NEDD9 immunostaining in human lung cancer specimens