Supplementary Figure 3 from Reciprocal Metabolic Reprogramming through Lactate Shuttle Coordinately Influences Tumor-Stroma Interplay

PDF file - 385K, 8.Lactate up-load by HPFs and PCa-AFs; 10000 HPFs were treated with serum-free medium or with CM-PCa for 24 hours (PCa-AFs). U-14Clactate were added to the culture media for 30 minutes, then cells were lysated and incorporated radioactive lactate were measured by scintillator. The obtained values were normalized on protein content.