Figure S1. Overexpression and knockdown of H1.3. from Histone H1.3 Suppresses <i>H19</i> Noncoding RNA Expression and Cell Growth of Ovarian Cancer Cells

Figure S1. Overexpression and knockdown of H1.3. A) Generation of OV-3/H1.3 stable cell lines. Western blotting of H1.3 overexpressing clones. OVCAR-3 cells were transfected with a pcDNA-FLAG-H1.3 construct. G418 resistant clones were screened by Western blotting using an anti-FLAG antibody. Coomassie staining shows equal loading of the cell lysates. Lane 1: OVCAR-3; Lane 2: OVCAR-3 transfected with pcDNA3 vector only (designated as OV-3/V.O); Lanes 3-9: OVCAR-3/H1.3 clones. Clones marked with "*" and "#" were used as representative high and low FLAG-H1.3 expressing clones (OV-3/H1.3(H) and OV-3/H1.3(L)). B) Knockdown of H1.3 levels by shRNA in OV-3/H1.3(H) cells. Whole cell lysates were analyzed by Western blotting using an anti-H1.3 antibody. Western blots using an anti-ACTIN antibody were included as loading controls.