Supplementary Figure 4 from Acidity Generated by the Tumor Microenvironment Drives Local Invasion

PDF file - 77K, Figure S4: Quantification of tumor growth. Images are shown for mouse tumor #23 on day 4 (green) and day 14 (red). Images were co-registered using the ring clip of the DWC as a fiducial. The centroid of the tumor on day 4 was calculated and radial lines were drawn every 22.5o of arc, beginning at the ring. Growth was calculated as the number of pixels that the day 14 tumor extended beyond the edge of the day 4 tumor using Definiens Developer XD�.