Supplementary Figure S1 from USP9X Downregulation Renders Breast Cancer Cells Resistant to Tamoxifen

Figure S1. ERα positive breast cancer cells are rescued from tamoxifen induced growth arrest by USP9X knockdown, but ERα negative breast cancer cells are not. Knockdown of USP9X rescues tamoxifen-induced growth arrest in T47D cells. T47D cells were infected with the shRNA against USP9X recovered from the initial screen, pRS-USP9X II or pRS-GFP as control. Cells were cultured for 4-6 weeks in the presence of 1μM 4OH-tamoxifen. When colonies appeared cells were fixed and subsequently stained. Plate confluency was determined by calculating the number of cell-covered pixels related to the total surface area.