Supplementary Figure 4 from Chk2 Phosphorylation of Survivin-ΔEx3 Contributes to a DNA Damage–Sensing Checkpoint in Cancer
crossref(2023)
摘要
PDF file - 93K, Multiparametric flow cytometry detection of GammaH2AX reactivity. HCT116 cells were transfected with vector, WT survivin (SVV), survivin-DeltaEx3 or Chk2 phosphorylation-defective survivin-DeltaEx3-Mut3 cDNA, treated with etoposide (A) or camptothecin (B), and analyzed after 16 h for GammaH2AX (y-axis) and PI (x-axis) staining by multiparametric flow cytometry. In panel A, numbers in parenthesis correspond to GammaH2AX fluorescence units after background subtraction.
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