Supplementary Video 1 from Identification of Two Distinct Carcinoma-Associated Fibroblast Subtypes with Differential Tumor-Promoting Abilities in Oral Squamous Cell Carcinoma

MP4 file - 752K, 1. Time-lapse microscopy of CAF1 plated onto collagen I showing the diversity of CAF movement, with a predominance of motile cells with longer tracks. Images were captured every 10 mins and analysed using IMARIS software. The centre of each CAF is marked by a grey square. Migration was visualized by a track that changes colour in time as shown by the scale lower right corner. Examples of highly motile CAF are shown in the left quadrants and less motile CAF at the junction of the upper and lower left quadrants. Quantification of the migration is shown in supplementary Table ST1.