Supplementary Figure 2 from Reciprocal Metabolic Reprogramming through Lactate Shuttle Coordinately Influences Tumor-Stroma Interplay

PDF file - 3.3K, Enrichment of glycolitic enzymes among genes up-regulated in prostatic CAFs; Gene expression profiles were analyzed using an Illumina microarray platform (unpublished data) on paired CAFs and HPFs isolated respectively from the tumor and the adjacent normal tissue from three prostate cancer patients subjected to radical prostatectomy. Gene Set Enrichment Analysis (GEA) (1,2 http://broadinstitute.org/gsea)was conducted between CAFs and HPFs, and revealed, among the others, a significant enrichment for gene sets related to carbohydrate catabolism among the genes that were up-regulated in CAFs. Specifically, when the analysis was carried out searching among gene ontology gene sets (C5), enrichment was found for Carbohydrate_Catabolic_Process set, whereas and ever higher enrichment was found for a set of glycolytic enzymes (referred to as Mootha_Glycolysis) when the analysis was performed on literature based gene sets (C2). (A) Table showing normalized enrichment scores and significance for enriched gene set. (B) Enrichment plot (left) and heatmaps of gene intensities (right) for the gene set emerged from GEMSA. In the heatmaps, gene intensities are colored from blue (low expression)to red (high expression). (1) Subramanian, Tamayo et al. (2005) PNAS 102, 15545-15550 (2) Mootha, Lindgren et al. (2003) Nat Genet 34, 267-273