Supplementary Figure 3 from Chronic Autophagy Is a Cellular Adaptation to Tumor Acidic pH Microenvironments

PDF file - 431K, Histological analysis of in-vivo LC3 expression in HS766T subcutaneous tumors buffered with sodium bicarbonate. (A) Whole cell lysates from HS766T cells cultured at neutral pH 7.4 or pH 6.7 for 48 hours were analyzed for LC3-II expression. (B) Positive pixel analysis was completed for LC3 staining on whole tissue cross sections from HS766T tumors treated with tap or NaHCO3. An overall significant decrease in total positive and strong positive LC3 pixels was observed in NaHCO3 treated samples. The data are plotted as the mean � standard deviation of three whole tumor cross sections from each treatment group. (C) A 5x magnification of representative tissue regions from HS766T tumors stained for LC3