Supplementary Figures 1 - 6, Tables 1 - 6 from Molecular Profiling of Tumor Cells in Cerebrospinal Fluid and Matched Primary Tumors from Metastatic Breast Cancer Patients with Leptomeningeal Carcinomatosis

PDF file - 2197K, Methods describing fluorescence in situ hybridization (FISH) analysis in circulating tumor cells (CSFTCs). Case studies involving genomic profiling of tumor cells in the cerebrospinal fluid (CSFTCs) in 7 patients. Fig. S1. Workflow for sample processing. Fig. S2. Study scheme. Fig. S3. Comparison of genomic aberrations between CSFTCs, breast primary tumors, and CTCs. Fig. S4. Comparison of genomic aberrations between CSFTCs and corresponding primary tumors (n=6 pairs). Fig. S5. Genomic profiling of CSFTCs from patients A) 108, B) 112, C) 4014, D) CSF1, E) CSF2, F) CSF5, and G) CSF7. Fig. S6. Serial aCGH profiling of CSFTCs. Table S1. Cell inputs and sample quality control (QC) assessment. Table S2. Mutation screening via Sequenom OncoCarta Panel. Table S3. 64-gene panel for Taqman(TM) Low Density Array (TLDA) RT-PCR analysis. Table S4. Comparison of genomic aberrations between CSFTCs vs. breast primary tumors. Table S5. Comparison of genomic aberrations between CSFTCs vs. CTCs. Table S6. Copy number alterations at the chromosome arm level in CSFTC and corresponding primary tumor (PT), axillary lymph node (LN) tissues or distant metastasis (MET), and circulating tumor cells from peripheral blood (PB).