Supplementary Figure 1 from Low PIP4K2B Expression in Human Breast Tumors Correlates with Reduced Patient Survival: A Role for PIP4K2B in the Regulation of E-Cadherin Expression

PDF file - 65K, A. Lysates from control (pLKO1) or PIP4k2B (shPIP4k2B) knockdown MCF10A cells were immunoblotted using the PIP4K2BP6 antibody. B. The PIP4K2BP6 antibody was immunodepleted by passing it through a column to which the P6 antigenic peptide was coupled. The antibody before or after, as indicated, was used to probe western blots of increasing levels of PIP4K2B .