Supplementary Figures 1 - 8 from Identification of Two Distinct Carcinoma-Associated Fibroblast Subtypes with Differential Tumor-Promoting Abilities in Oral Squamous Cell Carcinoma

PDF file - 2707K, Fig. S1. Phenotypical characterization of CAF, DAF and NF used in the study. Fig. S2. Expression of known gene expression markers of CAFs at mRNA level. Fig. S3. Validation of microarray data at the protein level. Fig. S4. Invasion of transformed human oral keratinocytes in 3D constructs depends on the type of fibroblasts embedded in the collagen biomatrix. Fig. S5. Quantification of growth factor synthesis by CAF and NF maintained in 2D cultures and 3D constructs. Fig. S6. Quantification of RhoA-GTPase and Rac1/2/3-GTPase in CAF-N and NF. Fig. S7. Visualization of the pattern of distribution of hyaluronic acid (HA) in patient samples and 3D constructs with alcian blue staining. Fig. S8. Quantification of HAS2 mRNA levels in HAS2 shRNA (h) lentiviral transduced CAF.