Supplementary Figures 1 - 8 from TRAF4 Is a Critical Molecule for Akt Activation in Lung Cancer

PDF file - 1503K, Supplementary Figure 1. Knockdown of TRAF4 reduces tumorigenic properties in a panel of human lung cancer cell lines. Supplementary Figure 2. Knockdown of TRAF4 inhibits EGF-induced translocation of Akt to the membrane. Supplementary Figure 3. TRAF6 is unnecessary for EGF-induced Akt activation in human lung cancer cells. Supplementary Figure 4. TRAF4, but not Skp2, is overexpressed and required for Akt activation in human lung cancer cell lines. Supplementary Figure 5. The Ring domain and TRAFc domain are required for TRAF4-mediated HaCaT cell malignant transformation. Supplementary Figure 6. Knockdown of Akt1 inhibits EGF-induced HaCaT cell transformation. Supplementary Figure 7. Knockdown of Akt1 reduces tumorigenic properties of human lung cancer cells. Supplementary Figure 8. Cell counting. Cells that used for glucose uptake and lactate production measurement were counted.