Supplementary Figure 2 from Chk2 Phosphorylation of Survivin-ΔEx3 Contributes to a DNA Damage–Sensing Checkpoint in Cancer

PDF file - 39K, siRNA targeting of survivin-DeltaEx3. A. MCF-7 cells stably transfected with survivin were left untreated (None) or transfected with control (Ctrl) non-targeting or survivin-DeltaEx3-directed siRNA and analyzed by RT-PCR for expression of WT survivin (SVV) or survivin-DeltaEx3 transcripts (left). Right, densitometric quantification of mRNA bands for SVV or survivin-DeltaEx3 mRNAs in siRNA-transfected cells. RU, relative units. B. MCF-7 cells transfected with the indicated siRNAs as in A were analyzed for DNA content by propidium iodide (PI) staining and flow cytometry. The percentage of cells in the G2/M or >G2/M fraction is indicated.