Supplementary Figure S2 from USP9X Downregulation Renders Breast Cancer Cells Resistant to Tamoxifen

Figure S2. QPCR validation of ChIP-seq data at 45 minutes ligand treatment. (A) Genome browser snapshots of ERα chromatin binding events at the RARA and NRIP1 loci. Cells were ligand treated for 48 hours, prior to fixation. shGFP (blue) and shUSP9X (red) conditions were tested. Tag count and genomic coordinates are shown. (B) ERα ChIP-QPCR validation of the genomic locations, indicated in A. Cells were ligand treated during 45 minutes prior to fixation. shGFP (white) and shUSP9X (black) conditions were tested. Enrichment over negative control region was assessed. Error bar shows SD values from triplicate measurements.