Supplemental Figures S1-S12 from Oncogenic Transformation Can Orchestrate Immune Evasion and Inflammation in Human Mesenchymal Stem Cells Independently of Extrinsic Immune-Selective Pressure

Supplemental Figures S1-S12. qPCR validation of genes expression change identified by microarray analysis of HLA class I APM (S1); Increased inhibitory capacity of transformed hMSC is independent of cell lines proliferation rates (S2); Sequential transformation increases hMSC capacity to affect T-cell activation (S3); Neoplastic transformation reduces the inducible IDO and T cells chemo-attractors expression in hMSC (S4); Transformation of hMSC decreases transcription of HLA class I alleles induced by IFN-γ signaling (S5); IL-1β expression increases in hMSC following sequential transformation (S6); Neoplastic transformation of hMSC does not yield cells lysis or apoptotic cell death (S7); Transformation of human MSC leads to a variation in the expression of IL-1β signaling components (S8); IL-1β expression does not influence the proliferation rate of transformed hMSC (S9); Loss of IDO, CXCL9 and CXCL10 inducible expression is unrelated to IL-1β expression on neoplastic hMSC (S10); IL-1β production is not responsible for impaired IFN-γ response on transformed hMSC (S11); IL-1β expression increases in human fibroblast following sequential transformation (S12).