Supplementary Figure 4 from Reciprocal Metabolic Reprogramming through Lactate Shuttle Coordinately Influences Tumor-Stroma Interplay

PDF file - 818K, MCT1 transporter is important for PCa cell growth; A) MCT1 transporter is up-regulated in PCa cells. PCa cells were cultivated alone or in co-colture with CAFs (3:1 CAFs:PCa cells proportion). Cells were then treated with CM-HPFs, CM-CAFs or serum-free medium for 24 hours and then MCT1 and MCT4 expression was valutated by immunoblot analysis. B)MCT1 silencing decreases the growth of PCa clones. MCT1 silencing was obtained by RNA interference in PCa cells. 48 hours after silencing, PCa cells were used to seed a co-colture with CAFs (5:1, CAFs:PCa cells proportion). PCa clones are counted after five, six and seven days and the mean are shown in the bar graph.