Supplementary Figures 1 - 11 from Prodigiosin Rescues Deficient p53 Signaling and Antitumor Effects via Upregulating p73 and Disrupting Its Interaction with Mutant p53

PDF file - 703K, The induction of p21 by prodigiosin at earlier time point (4 hr) in HOP92 cells (S1); Prodigiosin and compound R induce cell cycle arrest at early time point (S2); Prodigiosin does not induce γH2AX foci formation (S3); The higher concentration (1 μM) of prodigiosin or compound R induces cellular apoptosis at 72 hr treatment in HFF normal cells (S4); Dose-responsive curve of prodigiosin in HFF normal cells and SW480 cancer (S5); The effect of prodigiosin on the p53 pathway in normal cells (S6); The effect of prodigiosin on mutant p53 in SW480 cells, and the effect of prodigiosin on wild type p53, p73 and their target proteins in HCT116 cells (S7); The effect of prodigiosin on p63 in SW480 and HCT116 p53-/- cells (S8); High concentration (100 nM) of rapamycin does not disrupt the interaction of mutant p53 and p73 (S9); The bar graph indicates the quantitative data for Ki67 and TUNEL staining (S10); Body weight of nude mice following 5 mg/kg prodigiosin or DMSO treatment in HT29 xenografts and SW480 xenografts (S11).