Supplementary Figures 1 - 7 from Interleukin 21–Induced Granzyme B–Expressing B Cells Infiltrate Tumors and Regulate T Cells

PDF file - 182K, Supplemental Figure 1. B cells activated with IL-21 and anti-BCR secrete enzymatically active granzyme B. Supplemental Figure 2. Interleukin 21 in the absence of B cells does not suppress T cell proliferation. Supplemental Figure 3. GrB-secreting B cells do not induce apoptosis in T cells. Supplemental Figure 4. IL-10, CD25 and IDO are expressed on IL-21-induced GrB+ Breg, but not on GrB- B cells. Supplemental Figure 5. IL-2 retitration has no significant effect on the suppression of CD4+ T cell proliferation by GrB-secreting B cells. Supplemental Figure 6. IL-21-induced GrB secretion by B cells depends on TLR7 and TLR9 signaling. Supplemental Figure 7. B cells in the microenvironment of solid tumors sporadically express IL-10.