Figure S1 from CD8<sup>+</sup> T-cell–Mediated Immunoediting Influences Genomic Evolution and Immune Evasion in Murine Gliomas

Transgenic murine glioma model possesses increased CD4+ and CD8+ T-cell infiltration early in tumor development. (A) Representative bioluminescence activity of tumor captured 21 and 35 days post-tumor induction (left). Scatter plot showing an example of an ex vivo flow cytometry of the brain tumors using the CD4+ and CD8+ markers (right). (B) Bioluminescence was measured at seven time points between 21 and 63 days post-tumor induction (n=8/time point). (C) Kaplan-Meier survival curve illustrating the time points of flow cytometric analysis relative to initiation of tumor-related death (n=16). (D) Bar plots showing CD4+ (left) and CD8+ (right) T-cell tumor infiltration normalized against the contralateral brain hemisphere, and bioluminescence signal at 21 and at 35 days post-tumor induction (n=16). Data in (D) is shown as mean {plus minus} SEM. p= 0.0189 and p= 0.026 by unpaired studentÂ's t-test.