Supplemental Data from Pharmacological Inhibition of the Histone Lysine Demethylase KDM1A Suppresses the Growth of Multiple Acute Myeloid Leukemia Subtypes

Includes Supplementary Methods, Supplementary Tables and Figures and References Supplementary Figure 1: Structure and enzyme inhibitory activity of the KDM1A tool compounds. Supplementary Figure 2: CETSA shows thermal stabilization of KDM1A in intact cells following treatment with active KDM1A inhibitors. Supplementary Figure S3: Effects of RN-1 treatment on colony formation in methylcellulose. Supplementary Figure 4: KDM1A inhibition effects on the RUNX1-RUNX1T1 containing cell line SKNO-1. Supplementary Figure 5: Lack of global changes in histone H3K4 and H3K9 methylation following KDM1A inhibition. Supplementary Figure 6: KDM1A inhibitors affect KDM1A chromatin binding pattern in SKNO-1 cells. Supplementary Figure 7: Treatment of leukemia cells with a combination of KDM1A inhibitor RN-1 and EZH2 inhibitor CPI-169 leads to a synergistic cell killing in AML cell lines.