CRISPR/Cas9-Mediated Genome Editing in Zebrafish.

Jeffrey G Daniel,Xinge Yu,Allison C Ferguson,Jordan A Shavit

Methods in molecular biology (Clifton, N.J.)（2023）

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摘要

The CRISPR/Cas9 system is a powerful tool for genome editing in zebrafish. This workflow takes advantage of the genetic tractability of zebrafish and will allow users to edit genomic sites and produce mutant lines using selective breeding. Established lines may then be employed by researchers for downstream genetic and phenotypic analyses.

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关键词

CRISPR/Cas9,Genome editing,Mutagenesis,Zebrafish

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