Molecular Engineering of Benzenesulfonyl Analogs for Visual Hydrogen Polysulfide Fluorescent Probes Based on Nile Red Skeleton
Spectrochimica acta Part A, Molecular and biomolecular spectroscopy(2023)
摘要
Hydrogen polysulfide (H2Sn, n > 1) has a valuable function in various aspects of biological regulation. Therefore, it is of great significance to realize the visual monitoring of H2Sn levels in vivo. Herein, a series of fluorescent probes NR-BS were constructed by changing types and positions of substituents on the benzene ring of benzenesulfonyl. Among them, probe NR-BS4 was optimized due to its wide linear range (0 similar to 350 mu M) and little interference from biothiols. In addition, NR-BS4 has a broad pH tolerance range (pH = 4 similar to 10) and high sensitivity (0.140 mu M). In addition, the PET mechanism of probe NR-BS4 and H2Sn was demonstrated by DFT calculations and LC-MS. The intracellular imaging studies indicate that NR-BS4 can be successfully devoted to monitor the levels of exogenous and endogenous H2Sn in vivo.
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关键词
Nile red,Fluorescent probe,H2Sn,Cell imaging,Zebrafish imaging
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