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Analytical aspects of the antinuclear antibody test by HEp-2 indirect immunofluorescence: EFLM report on an international survey.

Martine Vercammen,Carolien Bonroy,Sylvia Broeders,Edward K L Chan,Nicola Bizzaro,Dimitrios P Bogdanos,Luis Andrade,Wim Coucke,Wilson de Melo Cruvinel,Ana Kozmar,Liisa Kuhi,Laurence Lutteri,Maria Jose Rego de Sousa,Sofie Schouwers,Lieve Van Hoovels,Xavier Bossuyt

Clinical chemistry and laboratory medicine（2023）

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Abstract
Even though many laboratories embrace high-quality HEp-2 IFA, substantial differences in how HEp-2 IFA is performed and controlled remain. Acting according to the EFLM/EASI/ICAP ANA recommendations can improve the global performance and quality of HEp-2 IFA and nurture harmonization.
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Key words
antinuclear antibodies,indirect immunofluorescence,survey
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