Supplementary Figures (Figure S1 - S7) from SHMT2 Desuccinylation by SIRT5 Drives Cancer Cell Proliferation

Figure S1 shows the SIRT5 interaction network and SHMT2 identification. Figure S2 shows that SIRT5 is irreplaceable for cancer cell in response to serine deprivation. Figure S3 shows that SIRT5 desuccinylates SHMT2. Figure S4 shows that SIRT5 upregulates SHMT2 enzymatic activity under metabolic stress. Figure S5 shows that K280 is the major succinylation site of SHMT2. Figure S6 shows that succinylation of SHMT2 at K280 restrains cellular redox balance and clonogenic growth. Figure S7 shows that succinylation of SHMT2 at K280 impairs tumor cell growth in vivo and in vitro