Supplementary Figures from Activation of MAPK Pathways due to DUSP4 Loss Promotes Cancer Stem Cell-like Phenotypes in Basal-like Breast Cancer

PDF file, 392K, Correlation of DUSP4 gene expression and DUSP4 copy number across 444 breast cancer samples (S1); Cytokine array of conditioned media from MDA-231 cells after DUSP4 knockdown (S2); Upregulation of IL6 and IL8 after sustained shDUSP4 expression in MDA-231 cells (S3); MEK pathway inhibition limits mammosphere formation and self-renewal in MDA231 cells (S4); Association of genomic DUSP4 loss with p-cJUN expression in the TCGA data (S5); Chromatin Immunoprecipitation of ETS-1 at the IL8 promoter (S6); Mammosphere quantification of SUM159PT cells in the presence of alternative MEK inhibitors (S7); Inhibition of MEK and JNK increases the CD24+ population in BLBC cells (S8); Escape from long term enforced expression of DUSP4 in SUM159PT/pINDUCER-DUSP4 cells (S9).