Supplementary information from The STAT3-miRNA-92-Wnt Signaling Pathway Regulates Spheroid Formation and Malignant Progression in Ovarian Cancer

Table S1. Target sequence of STAT3 shRNAs Table S2. Primers sequence of miR-92a and pre-miR-92a QPCR Table S3, Clinicopathologic characteristics of patients with epithelial ovarian cancer Fig.S1. STAT3 silencing by shRNA in EOC cell lines inhibits the cell viability in 3-dimensional cultures. Fig.S2. Immunoblot analysis of SKOV3/SKOV3 STAT3-KD cells for the expression of cleaved-PARP, Bcl-xL, and xIAP. b-actin was used as a loading control. Fig.S3. Top, representative spheroids formed by ES2 scramble/ES2 STAT3-KD cells treated with or without paclitaxel (PTX). Bottom, plot of the number of spheroids formed per 1000 cells in selected cells. Error bars represent the SD from triplicate cultures.