Supplementary Movie 4 from β1 Integrin Targeting Potentiates Antiangiogenic Therapy and Inhibits the Growth of Bevacizumab-Resistant Glioblastoma

MOV file - 868K, Supplementary Movie 4. Inhibitory Anti-Beta1 Integrin mAb OS2966 Disrupts BRG3 Adhesion, Spreading, And Migration. Related to Figure 2. Timelapse phase-contrast imaging of BRG3 bevacizumab-resistant cells on fibronectin demonstrates mesenchymal migration of control (10 μg/ml IgG, left) cells into the wound in a scratch wound healing assay. In contrast, BRG3 cells in the treatment condition (10μg/ml OS2966, right) rapidly lose adhesive contacts to the substrate (within 20-30 mins) and are unable to migrate into the wound. Movie images captured with a 40x objective representing approximately 10 hours of imaging, 35 minutes between each frame.